Valued at USD 649.42 million in 2024, the U.S. next-generation sequencing library preparation market is poised for substantial expansion, with a CAGR of 12.83% anticipated between 2025 and 2034. This growth is being propelled by a confluence of technological innovation, increasing demand for targeted sequencing panels, and the integration of artificial intelligence in genomic data interpretation. A segmentation-driven analysis reveals significant variation in performance across product types, end-user industries, and application areas, with each segment responding uniquely to evolving clinical and research demands. As the